Increased expression of chondrogenic markers will be Evaluated by different techniques:
in vitro: Histology fit for chondrogenic markers, RT-PCR on the following markers aggrecan, type II collagen, Sox9, Comp, type IX collagen
in vivo: In a second step, differentiated MSCs are implanted in vivo after combination with a hydrogel subcutaneously in nude mice. The formation of a neo cartilage tissue will be assessed by histology for type II collagen and aggrecan
Read more: http://www.clinicaltrials.gov/ct2/show/NCT01879046?cond=%22Arthritis%22&lup_s=08/23/2013&lup_d=30
